Mahidol University Annual Research Abstracts
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Microsoft Faculty of Medicine Siriraj Hospital
Another form of cirrhosis, primary biliary cirrhosis (PBC), damages the bile ducts in the liver, and occurs primarily in women over 35 years of age.
Laptop Computers lected from paraffin blocks deposited at the Department of Pathology, Siriraj Hospital was screened for the presence of the predominant form of IGFs (IGF-I or -II). Tumor tissues that express any specific form of IGFs more than adjacent wild-type cells are potential candidates for an antisense gene therapy against the expression of that specific form of IGF. Paraffin sections containing these cancer cells and adjacent wild-type cells were examined by immunohistochemical technique using mouse IgG-1 antihuman IGF-I, or antihuman IGF-II as primary antibodies. Normal liver tissue, known as the largest producer of both forms of IGFs, was used as a positive control. The staining pattern of both IGF-I and IGF-II in the liver was highest in the cytoplasm of normal hepatocytes. The majority of the tumors except for papillary thyroid carcinoma and nasopharyngeal carcinoma strongly expressed IGF-I. Only a few samples of the tissues studied expressed a low level of IGF-II, if any. This raises a further working proposal that the blockade of IGF-I synthesis in these tumors may help to delay the tumor progression so that an effective immune response can be established and destroy the tumor.
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Laptop Computer (Siriraj Hosp Gaz 2000; 52: 471-476.)
- Price of the plan is based on the total sales price of the desktop and peripherals. (1) Single Peripheral (single printer, single scanner); Only one laptop or desktop computer per contract; Multiple brands covered per contract; • Laptop Repair To initiate a laptop repair, a box is express shipped to your location, express shipped to a certified repair facility, and then express returned to you.
Desktop Computer
Notebooks
Lenovo PRIMARY CULTURES OF CHOLANGIOCARCI-NOMA ESTABLISHED FROM
BILE FLUID EXPRESS INSULIN-LIKE GROWTH FACTOR I AND FAS (NO. 403)
Hard Drive
Travelstar Adisak Wongkajornsilp, Yudhtana Sattawatthamrong, Sukit Huabprasert
Gateway Department of Pharmacology and Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University.
Laptop Parts Key words : Cholangiocarcinoma, primary culture
Software Cholangiocarcinoma cells were isolated from biliary fluid aspirated from the lumen of bile duct upstream from the intrahepatic obstruction in subjects inflicted with cholangiocarcinoma using endoscopic retrograde cholecystopancreatomy (ERCP). These established cultured cells were confirmed for their epithelial origin by their intensely staining with primary antibody against cytokeratin. By using flow cytometric analysis, these cultured cell were positively stained for insulin-like growth factor 1 (IGF-1) and Fas (CD95) using the respective specific monoclonal antibodies. The findings of IGF-1 expression suggested that IGF-1 could mediate an autocrine action in the proliferation of these cells. The downregulation of IGF-1 within these cells may have a therapeutic potential in the respective subjects. The observation of Fas on these cells suggested that these cancer cells could enter into apoptotic stage in responsive to contact with Fas ligand (CD95L).
Hard Drives (Thai J Pharm 2000; 22(Sup.1)S85.)
Electronics
Canon
Desktop Pc THE CONSTURCTION OF NOVEL EPISOMES TRANSCRIBING A mRNA OF FAS
AND AN ANTISENSE RNA OF FAS LIGAND FOR TRANSFECTION IN
CULTURED TUMOR CELLS (NO. 404)
Desktop Computers
Think Pad Adisak Wongkajornsilp1, Valla Wamanutajinda1, Sukit Huabprasert1, Voravut Chanyavanich2, Nantasak Tisavipat2 1Department of Pharmacology, and 2Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University.
Repair Key words : Fas, FasL
Data Recovery The expression of Fas ligand (FasL) on the surface of tumor cells has been proposed as a mechanism in which tumor cells evade cell-mediated immune responses. The direct interaction between FasL and Fas, the specific receptor on the surface of target cells, will activate a set of endogenous mediators and eventually lead to apoptosis within target cells. Upon direct contact with FasL on tumor cells, T cells that harbor Fas on their cell surface can undergo apoptosis. Hence the population of tumor-specific T cells within the proximity of tumor cells was controlled by such mechanism of contact inhibition. We proposed that the suppression of FasL expression or the overexpression of Fas in tumor cells could improve the autogeneic immune response against the tumor cells. This hypothesis warrants the construction of separate expression of systems for the antisense RNA of FasL and for the mRNA of Fas. We have synthesized two novel episomes, pAnti-FasL and pFas that can express the antisense FasL mRNA and the Fas mRNA within eukaryotic cells. Both episomes were modeled after pAnti-IGF-1, the episome that expresses the antisense RNA of IGF-1 upon the activation with ZnSO4. The pAnti-IGF-1 has been used in gene therapy against cancer. The cDNA construct of either FasL or Fas obtained from Dr. Shigekazu Nagata was subcloned to replaced the IGF-1 sequence in antisense and sense orientation respectively within the pAnti-IGF-1. Both novel episomes were successfully constructed. The expression of the antisense RNA of FasL from pAnti-FasL and the expression of Fas mRNA were controlled by the addition of ZnSO4. The function of both pAnti-FasL and pFas in transfected cultured tumor cells were confirmed using flow cytometry with the respective specific monoclonal antibodies. These episomes are crucial in the study for the dominance of tumor-derived immunosuppression based on contact inhibition. Such in vitro evidence of improved anti-cancer immune response could warrant the use of both episomes for gene therapy. Both episomes contain all components necessary for clinical application since they are derived from pAnti-IGF-1, the episome already approved for such purpose.
Cisco
Keyboard
Monitor INFORMATION TECHNOLOGY MASTER PLAN OF SIRIRAJ HOSPITAL AND
ITS PERSONNEL (NO. 405)
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Infosys Chailerd Pichitpornchai
Refurbished Laptops Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10900
Wipro The Information Technology (IT) has been used at the Faculty of Medicine Siriraj Hospital to implement 3 main computer systems: (1) Siriraj Hospital Information System (SiHIS), (2) Siriraj Internet through Mahidol University Campuses Network (SiMUC-Net), and (3) Siriraj Intranet (SiNet) and they have been on service for over 5, 2, and 0.5 years, respectively. The ultimate purpose of all computer systems is to provide medical education, research, medical service, and the Faculty of Medicine and the hospital administration. However, at present each of the computer systems has not reached all the mentioned roles. It is this IT master plan that describes how all the computer systems will achieve their full roles in the next 3 years.
Lap Top Prior to further develop the 3 computer systems, research and development methodology must be used to identify the IT re-
Refurbished
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