Protein amplification in melanoma is possible drug target
July 11, 2005
Laptop Battery Researchers have pinpointed specific gene and protein
over-production in metastatic melanoma, pointing the way to a
possible new drug target, according to a study published in Nature
July 7.
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Thinkpad Gene amplification is a process that often happens in cancer
cells when the normal DNA replication process is altered, causing
many copies of the gene to be produced instead of a single copy of
a region of a chromosome.
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Microsoft The researchers found that the MITF (microphthalmia-associated
transcription factor) master regulator is the target of gene
amplification in melanoma. MITF amplification also was more
prevalent in metastatic disease and was correlated with decreased
overall patient survival.
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Laptop Computers Collaborators at Yale, using the technology AQUA (Automated
Quantitative Analysis) to quantitatively measure protein expression
in melanoma tissue microarrays, also found an over-expression of
the protein.
The remaining sediments were used in PCR amplification based on IS6110 as a DNA target, and in antigen detection by dot immunoassay (DIA).
Laptop Computer "According to the study, these data suggest that MITF represents
a distinct class of 'lineage survival' or 'lineage addiction'
oncogenes required for both tissue-specific development and tumor
progression," said David Rimm, M.D., an author on the study and
associate professor in the Department of Pathology at Yale School
of Medicine.
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Desktop Computer Another Yale author on the study was Aaron Berger, a student in
Rimm's laboratory. The corresponding author was William Sellers of
Dana-Farber Cancer Institute in Boston. Co-authors included
researchers at Brigham and Women's Hospital, Harvard, the
Massachusetts Institute of Technology, Massachusetts General
Hospital, and the Medical University of Vienna.
Notebooks The AQUA technology is the property of HistoRx, Inc., a New
Haven-based bioscience company offering novel digital technologies
for in situ diagnostics developed at Yale School of Medicine. It
enables researchers to localize and quantify proteins in tissue
while maintaining spatial relationships-a process that was
previously impossible with conventional methods of pathology
analysis and which vastly increases the quality and amount of
information for analysis.
Lenovo Yale University
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